Analysis of Ser aminoacyl synthetase.

Analysis performed on 2024-07-10 11:39:42

Number of Proteins Analyzed

Initial Filtered Out Final Blast
Bacteria 42718 523 42195 26446
Archaea 3027 81 2946 1257
Vertebrata 1192 357 835 411
Mammals 577 90 487 134

Number of Variants Analyzed:

Protein Length Pathogenic Variants Benign/Unknown Variants
514 9 285

Domains

Domains
Current Study 484, 515
Guo et al (2010) 461, 514

Results

Mutations in Ancient vs Modern Domains

Current Study

Ancient Domains Modern Domains All Ratio
Number of Residues 482 32 514 15.0625
Number of Variants 274 20 294 13.7
Number of Pathogenic Variants 9 0 9 Undefined
Number of Benign/Unknown Variants 265 20 285 13.25
Fisher exact test 1.0

The Fisher exact test was calculated using a contingency table with the number of pathogenic and benign/unknown variants in ancient and modern domains. The bottom left values.

Guo et al (2010)

Ancient Domains Modern Domains All Ratio
Number of Residues 460 54 514 8.518519
Number of Variants 254 40 294 6.35
Number of Pathogenic Variants 9 0 9 Undefined
Number of Benign/Unknown Variants 245 40 285 6.125
Fisher exact test 0.615238

The Fisher exact test was calculated using a contingency table with the number of pathogenic and benign/unknown variants in ancient and modern domains. The bottom left values.

Coverage of the alignment and domain locations

Agreement between the current study and Guo et al (2010)

Conservation of the amino acids in modern and ancient domains

# of sites Ancient # sites Modern Mean Ancient (SD) Mean Modern (SD) Median Ancient Median Modern Mann-Whitney U p-value
Current Study 482 32 0.89 (0.01) 0.73 (0.03) 9.251e-01 6.803e-01 11422.00 2.56e-06
Guo et al (2010) 460 54 0.89 (0.01) 0.78 (0.02) 9.256e-01 8.147e-01 17071.00 3.33e-06

The Mann-Whitney U test was calculated using the conservation scores of the amino acids in ancient and modern domains. The p-value is for the null hypothesis that the conservation scores in ancient domains are greater than in modern domains.

Conservation of the amino acids in modern and ancient domains